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Rapid Plasma Reagin (RPR)
Principle
The RPR Card Test is a non-treponemal screening assay used for the serologic detection of syphilis. It is recommended when venous blood collection is performed. In this method, a carbon-particle cardiolipin antigen reacts with “reagin,” an&
Specimen Requirements
Specimen
Collect 2 mL of serum in a red-top tube (no anticoagulant).
Collection
Separate serum from cells as soon as possible to prevent hemolysis.
Storage and Transport
- 2–8 °C: use within 48 hours
- −80 °C: required if testing is delayed beyond 48 hours
Plasma
- Collect blood in EDTA anticoagulant tubes.
- Keep plasma in the original collection tube and centrifuge before use.
- Storage:
- 2–8 °C: use within 48 hours
Method
Agglutination Test.
Normal Range
Non-reactive
Turnaround Time
One Week
References
- Manual of test for syphilis, PHS Publication No. 411; 1969.
- Bender, et al. Clinical Laboratory Procedure Manual, NCCLS. Code GP2-A, Vol. 4, NO. 2.