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Platelet Auto-antibodies

Principle

In conditions associated with thrombocytopenia, platelet destruction may be mediated by auto-antibodies directed against platelet surface antigens. Although various methods such as radioimmunoassay and enzyme-based assays can detect platelet-associated

Specimen Requirements

Specimen

Whole blood

Collection

Collect 20 mL of blood in a heparinized tube.

Storage and Transport

  • Send at room temperature
  • Do not refrigerate
  • Deliver to the laboratory within 24 hours

Handling

Mix gently after collection to prevent clotting.

Method

Flow Cytometry

Normal Range

Negative
Reported as negative or positive

Turnaround Time

3 business days

References

  • Lazarchick, J. and Hall, S.  Platelet-associated IgG assay using flow cytometric analysis.  J. Immunol.  Meth. 87:275, 1986.