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HIV-1/2 Antibody Plus Antigen-Fourth Generation

Principle

This assay is an in vitro Enzyme-Linked Immunosorbent Assay (ELISA) designed for the simultaneous qualitative detection of antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2), as well as HIV-1 p24 antigen, in human serum. A react

Specimen Requirements

Specimen

Serum

Collection

Collect 2 mL of serum in a red-top tube (no additives) or a serum separator tube (gel barrier).

Processing

Separate serum from the clot as soon as possible to avoid hemolysis.

Storage and Transport

  • 2–8 °C: up to 1 week
  • −20 °C or lower: for longer storage
  • Avoid repeated freeze–thaw cycles

Method

Enzyme-Linked Immunosorbent Assay (ELISA).

Normal Range

Non-reactive

Turnaround Time

One Week

References

  • Feorino, P.M., Jaffe, H.W., Palmer, E. et al. Transfusion-associated acquired immunodeficiency syndrome: evidence for persistent infection in blood donors. New Engl J Med 312 (20):1293-6; 1985.
  • Sheehan, C. Clinical Immunology: Principl