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Chemistry Panels

Principle

VITROS chemistry slides are dry, multilayered analytical elements coated on polyester supports. A small volume of patient sample is applied to the slide and evenly distributed through the layers. The spreading layer contains the appropriate substrates and reagents required for the reaction. The analyte in the sample catalyzes a reaction sequence producing products that absorb light at specific wavelengths (340–680 nm). The products diffuse into underlying layers and are measured by reflectance spectrophotometry. Test methodologies include colorimetric, enzymatic endpoint, two-point or multipoint rate, and potentiometric assays. The rate of change in reflection density or amount of colored complex formed is proportional to the analyte concentration in the sample.

Specimen Requirements

Specimen

Serum (red-top tube with serum separator, SST)

Collection

Allow blood to clot completely, then centrifuge promptly to separate serum and prevent hemolysis.

Processing

Allow blood to clot completely, then centrifuge promptly to separate serum and prevent hemolysis.

Storage and Transport

Transport at room temperature (15–30°C) if sent to the laboratory the same day. If testing is delayed, centrifuge and refrigerate at 2–8°C.

Unacceptable Specimens

Hemolyzed and/or markedly lipemic specimens, which may interfere with certain chemistry assays.

Method

Dry Slide Chemistry (VITROS)

Normal Range

See individual test report.

Basic Metabolic Panel

  • Glucose
  • Calcium
  • BUN
  • Creatinine
  • BUN/Creatinine Ratio
  • Sodium
  • Potassium
  • Chloride
  • Bicarbonate (ECO₂)

Comprehensive Metabolic Panel 

  • Glucose
  • Total Protein
  • Albumin
  • Globulin
  • A/G Ratio
  • Total Bilirubin
  • ALT (SGOT)
  • AST (SGPT)
  • Alkaline Phosphatase
  • Calcium
  • BUN
  • Creatinine
  • BUN/Creatinine Ratio
  • Sodium
  • Potassium
  • Chloride
  • Bicarbonate (ECO2)

Lipid Panel

  • Triglycerides
  • Total Cholesterol
  • HDL Cholesterol
  • CHOL/HDLC
  • LDL
  • VLDL

Electrolyte Panel

  • Sodium
  • Potassium
  • Chloride
  • Bicarbonate (ECO₂)

Hepatic Function Panel

  • Albumin
  • Total Bilirubin
  • Direct Bilirubin
  • Alkaline Phosphatase
  • AST (SGPT)
  • ALT (SGPT)

Turnaround Time

2 days

References

  • Johnson & Johnson Clinical Diagnostics, Inc. VITROS Chemistry Products: Test Methodology. August 1997.

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