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CBC with Differential
Principle
The complete blood count (CBC) with 5-part differential is a comprehensive hematologic assay that quantifies circulating red blood cells, white blood cells, and platelets, along with several erythrocyte indices. The test includes measurement of red blood cell count (RBC), hemoglobin (Hgb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), platelet count, and white blood cell count (WBC). The 5-part differential provides both absolute and relative counts of neutrophils, lymphocytes, monocytes, eosinophils, and basophils.CBC) with 5-part differential is a comprehensive hematologic assay that quantifies circulating red blood cells, white blood cells, and platelets, along with several erythrocyte indices.
This assay is used to evaluate anemia, infection, inflammation, immune disorders, hematologic malignancies, and other conditions affecting blood cell production or survival.
Specimen Requirements
Specimen
Collection
Processing
Storage and Transport
Keep and transport at room temperature.
Unacceptable Specimens
- Specimens collected in tubes other than EDTA
- Tubes less than half full
- Improperly labeled specimens
- Hemolyzed specimens
- Specimens older than 48 hours
- Clotted specimens
Method
VCS Technology and Hemoglobinometry.
Normal Range
See lab report.
Turnaround Time
References
- Bender et al. Clinical Laboratory Procedure Manual, NCCLS Code GP2-A. Vol. 4, No. 2.
- Coulter MAXM Reference Manual. Coulter Corporation; 1995.
- Coulter MAXM Operator’s Guide. Coulter Corporation; 1995.
- Coulter MAXM S-CAL Kit Package Insert; 1985–1993.
- Coulter MAXM 5C Plus Coulter Cell Control Insert.
- Lotspeich-Steininger C, Stiene-Martin EA, Koepke JA. Clinical Hematology: Principles, Procedures, Correlations. Philadelphia, PA: J.B. Lippincott; 1992.
- Coulter MAXM Special Procedures and Troubleshooting. 1995.
- O’Connor BH. A Color Atlas and Instructional Manual of Peripheral Blood Cell Morphology. Baltimore, MD: Williams & Wilkins; 1984.