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Anti-Thyroid Antibodies Panel

Principle

Circulating thyroid autoantibodies are widely implicated in the etiology of autoimmune thyroid diseases. Both thyroglobulin (TG) and thyroid peroxidase (TPO) autoantibodies are routinely measured in clinical practice. Serum autoantibodies to thyroid mi

Specimen Requirements

Specimen

Serum

Collection

Collect one 10 mL red-top tube (no anticoagulant or preservative) by standard venipuncture.

Processing

Allow blood to clot and centrifuge at 1600 rpm for 10 minutes to separate serum.

Storage and Transport

  • Room temperature (15–30°C): up to 8 hours
  • 2–8°C: up to 48 hours
  • –20°C or below: for longer storage
    Send frozen serum on dry ice. Do not freeze samples in self-defrosting freezers. Avoid repeat

Minimum Volume

100 µL of serum

Method

Enhanced Chemiluminescence.

Normal Range

  • Anti-TPO: < 9 IU/mL
  • Anti-TG: < 4.0 IU/mL

Turnaround Time

3 days

References

  1. Davies TF and DeBernardo E, Thyroid autoantibodies and disease. In “Autoimmune Endocrine Disease”, TF Davies ed, Wiley, New York, NY, 1983, p. 127.
  2. Wall JR and Kuroki T. Immunologic Factors in thyroid disease. Medical Clini