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Anti-Phospholipid Antibodies Panel
Principle
The presence of antibodies to phospholipids—particularly cardiolipin—has been correlated with several diseases. Sensitive assays for various anti-phospholipid antibodies (with anti-cardiolipin antibodies being the most frequently measured) have revealed their clinical association with thromboembolic disease, thrombocytopenia, systemic lupus erythematosus (SLE), and recurrent spontaneous abortions (fetal loss). The coexistence of a biologic false-positive test for syphilis, lupus anticoagulant, and anti-cardiolipin antibodies has also been demonstrated. Detection of these antibodies using a solid-phase Enzyme-Linked Immunosorbent Assay (ELISA) provides a sensitive and semi-quantitative method for clinical evaluation.
Specimen Requirements
Specimen
Collection
Storage and Transport
- If testing or shipping within 24 hours: Keep at room temperature.
- If delayed: Centrifuge clotted blood at 1600 rpm for 10 minutes, separate the serum, and freeze at -70°C or below.
- Do not freeze samples in self-defrosting
Handling
Method
Enzyme-Linked Immunosorbent Assay (ELISA).
Normal Range
Negative
Results are reported as negative, borderline,positive or high positive
Turnaround Time
Anti-phospholipid antibody panel includes
- Anti-cardiolipin antibody
- Anti-phosphatidylserine antibody
- Anti-phosphatidylethanolamine antibody
- Anti-phosphatidic acid antibody
- Anti-phosphatidyl-glycerol antibody
- Anti-phosphatidyl-inositol an
References
- Miyakis S, Lockshin M.D., Atsumi T., et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). Journal of Thrombosis and Haemostasis. 2006;4(2):295–306.