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Anti-Phospholipid Antibodies Panel

Principle

The presence of antibodies to phospholipids—particularly cardiolipin—has been correlated with several diseases. Sensitive assays for various anti-phospholipid antibodies (with anti-cardiolipin antibodies being the most frequently measured)&

Specimen Requirements

Specimen

Serum

Collection

2 mL of serum collected in a red-top tube (no additive) or serum separator tube (gel barrier).

Storage and Transport

  • If testing or shipping within 24 hours: Keep at room temperature.
  • If delayed: Centrifuge clotted blood at 1600 rpm for 10 minutes, separate the serum, and freeze at -70°C or below.
  • Do not freeze samples in self-defrosting

Handling

Keep at room temperature or frozen as specified below. Serum may be separated from the clot. Minimum volume: 200 µL.

Method

Enzyme-Linked Immunosorbent Assay (ELISA).

Normal Range

Negative
Results are reported as negative, borderline,positive or high positive

Turnaround Time

7-10 business days

Anti-phospholipid antibody panel includes

  • Anti-cardiolipin antibody
  • Anti-phosphatidylserine antibody
  • Anti-phosphatidylethanolamine antibody
  • Anti-phosphatidic acid antibody
  • Anti-phosphatidyl-glycerol antibody
  • Anti-phosphatidyl-inositol an

References

  1. Miyakis S, Lockshin M.D., Atsumi T., et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). Journal of Thrombosis and Haemostasis. 2006;4(2):295–306.