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Anti-Extractable Nuclear Antibodies Panel
Principle
Each rheumatic disease typically exhibits a characteristic antinuclear antibody (ANA) profile. Therefore, identifying the specific ANA pattern in a patient’s serum is valuable for the differential diagnosis of autoimmune connecti
Specimen Requirements
Specimen
Serum
Collection
Collect one 10 mL red-top tube (no anticoagulant or preservative) by standard venipuncture.
Processing
Allow blood to clot and centrifuge at 1600 rpm for 10 minutes to separate serum.
Storage and Transport
- Room temperature: up to 8 hours
- 2–8°C: up to 48 hours
- –20°C or below: for longer storage
Send frozen serum on dry ice. Do not freeze samples in self-defros
Minimum Volume
100 µL of serum
Method
Enzyme-Linked Immunosorbent Assay (ELISA)
Normal Range
Negative.
Positive results for each ENA antigen are indicated separately.
Turnaround Time
7-10 business days
Panel includes
- Anti-ENA includes Sm and RNP
- Anti-Sjögrens includes SSA and SSB
References
Kądziela, M., et al. The Art of Interpreting Antinuclear Antibodies (ANAs) in Clinical Practice,
Journal of Clinical Medicine. 2025.