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Anti-DNA Antibodies Panel

Principle

Detection of antinuclear antibodies (ANA) against DNA and histones is an important diagnostic tool in the evaluation and management of rheumatic diseases. These autoantibodies react with various nuclear components, including polynucleotides, histones, nonhistone proteins, nucleolar antigens, and nuclear matrix compounds. Certain anti-DNA antibodies are considered marker antibodies for specific inflammatory rheumatic diseases, and different disease subsets may display distinct DNA antibody profiles. This Enzyme-Linked Immunosorbent Assay (ELISA) assay detects antibodies to the autoantigens double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), histones, and Scl-70 in human serum.

Specimen Requirements

Specimen

Serum

Collection

Collect one 10 mL red-top tube (no anticoagulant or preservative) by standard venipuncture.

Processing

Allow blood to clot and centrifuge at 1600 rpm for 10 minutes to separate serum.

Storage and Transport

Room temperature: up to 8 hours
2–8°C: up to 48 hours
–20°C or below: for longer storage

Send frozen serum on dry ice. Do not freeze

Minimum Volume

100 µL of serum

Method

Enzyme-Linked Immunosorbent Assay (ELISA)

Normal Range

Negative
Results are reported as negative, borderline or positive.

Turnaround Time

7-10 business days

Panel includes antibodies to

dsDNA
ssDNA
Histones
Scl-70

References

Reichlin M. Autoantibodies to nuclear antigens in systemic lupus erythematosus and related diseases. Rheumatic Disease Clinics of North America. 1992;18(2):369–387.
Mahler M, Fritzler M.J. The clinical significance o

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